Application of amplified fragment length polymorphisms markers to study the hybridization between Pinus sibirica and P. pumila
Keywords:interspecies hybridization, five-needle pines, Pinus sibirica, Pinus pumila, amplified fragment length polymorphisms, AFLP, genetic variation
AbstractThe genetic diversity of Pinus sibirica, P. pumila, and their hybrids, in a mixed natural stand was studied using amplified fragment length polymorphisms (AFLP). The stand was situated in the northern slope of the Khamar-Daban Ridge. Hybrid trees were determined by their morphological traits in the field. Three AFLP primer combinations detected 167 bands in 35 putative hybrids, and in 25 P. sibirica and 26 P. pumila individuals. Genetic variation in the hybrids was the highest, and was the lowest in P. sibirica. Principal coordinate analysis clustered P. sibirica, P. pumila and the hybrid genotypes into three distinct groups. Nei’s distance between the species was 0.193, whereas that between the hybrids and P. sibirica was 0.047, and between hybrids and P. pumila it was 0.074. Application of AFLP in the future studies promises to increase our knowledge of P. sibirica and P. pumila hybridization.
AndersonE., 1953. Introgressive hybridization. Biological Reviews. 28(3): 280–307.DOI: 10.1111/j.1469-185X.1953.tb01379.x Bensch S., Åkesson M., 2005. Ten years of AFLP in ecology and evolution: why so few animals? Molecular Ecology. 14: 2899–2914. DOI: 10.1111/j.1365-294X.2005.02655.x Chauhan N., Negi M. S., Sabharwal V., Khurana D. K., Lakshmikumaran M. 2004. Screening interspecific hybrids of Populus (P. ciliate × maximowiczii) using AFLP markers. Theoretical and Applied Genetics. 108: 951–957. DOI: 10.1007/s00122-003-1508-5 Galaziy G. I., 1954. Timberline flora in the mountains ofEastern Siberiaand its dynamics. Proceedings of Botanical Institute,USSRAcademyof Science 3(9): 210–329 (In Russian). Goroshkevich S. N., 1999. On the possibility of natural hybridization between Pinus sibirica and Pinus pumila in the Baikal Region. Botanicheskij Journal 84(9): 48–57 (In Russian). Goroshkevich S. N., Popov A. G., Vasilieva G. V., 2008. Ecological and morphological studies in the hybrid zone between Pinus sibirica and Pinus pumila. Annals of Forest Research 51: 43–52. Liu G. -F., Dong J. -X., Jiang Y., Lu Y. -F., Jiang J., Zhao G. -Y., 2005. Analysis of genetic relationship in 12 species of Section Strobus with ISSR markers. Journal of Forest Research. 16: 213–215. DOI: 10.1007/BF02856817 Miller L. M., 2000. Classifying genealogical origins in hybrid populations using dominant markers. Journal of Heredity. 91: 46–49. DOI: 10.1093/jhered/91.1.46 Molozhnikov V. N., 1975. Siberian dwarf pine in mountain landscape of Northern Baikalregion. Moscow, Nauka. 203 p. (In Russian). Nei M., 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics. 89: 583–590. Ostrowska E., Muralitharan M., Chandler S., Volker P., Hetherington S., Dunshea F., 1998. Optimizing conditions for DNA isolation from Pinus radiata. In Vitro Cellular and Developmental Biology–Plant. 34: 108–111. DOI: 10.1007/BF02822773 Peakall R., Smouse P. E., 2006. GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes. 6: 288–295. DOI: 10.1111/j.1471-8286.2005.01155.x Petrova E. A., Goroshkevich S. N., Belokon M. M., Belokon Y. S., Politov D. V., 2008. Population genetic structure and mating system in the hybrid zone between Pinus sibirica Du Tour and P. pumila (Pall.) Regel at the eastern Baikal Lakeshore. Annals of Forest Research. 51: 19–30. Petrova E. A., Goroshkevich S. N., Belokon M. M., Belokon Y. S., Politov D. V., 2012. Natural hybridization Siberian stone pine (Pinus sibirica Du Tour) and Siberian dwarf pine (P. pumila (Pallas) Regel) in southern Zabaikalie. Khvoynyye borealnoy zony. 30(1): 152–156 (In Russian). Petrova E. A., Goroshkevich S. N., Politov D. V., Belokon M. M., Popov A. G., Vasilyeva G. V., 2007. Seed production and genetic structure of populations in natural hybrid zone of Siberian stone pine and Siberian dwarf pine. Khvoynyye borealnoy zony. 24: 329–335 (In Russian). Politov D. V., Belokon M. M., Maluchenko O. P., Belokon Y. S., Molozhnikov V. N., Mejnartowicz L. E., Krutovsky K. V., 1999. Genetic evidence of natural hybridization between Pinus sibirica Du Tour and P. pumila (Pall.) Regel.ForestGenetics. 6: 41–48. Politov D. V., Krutovsky K.V., 2004. Phylogenetics, genogeography and hybridization of five-needle pines in Russia and neighboring countries. In: Breeding and genetic resources of five-needle pines: growth, adaptability, and pest resistance. Proceedings of the IUFRO Five-Needle Pines Working Party Conference, July 23-27, 2001, Medford, Oregon, USA, pp 85–97. Pozdnyakov L. K., 1952. Tree-like form of Siberian dwarf pine. Botanicheskij Journal 37(5): 688–691 (In Russian). Samils B., LagercrantzU., Lascoux M., GullbergU., 2001. Genetic structure of Melampsora epitea populations in Swedish Salix viminalis plantations. European Journal of Plant Pathology. 107: 399–409. DOI: 10.1023/A:1011270315251 Stebbins G. L., 1959. The role of hybridization in evolution. Proceedings of the American Philosophical Society. 103: 231–251. Vasilyeva G. V., 2011. Seed production and seed progeny growth of natural hybrids between Siberian stone pine (Pinus sibirica Du Tour) and Siberian dwarf pine (P. pumila (Pall.) Regel). Ph.D. thesis.Tomsk. 24 p. (In Russian). Vos P., Hogers R., Bleeker M., Reijans M., Van de Lee T., Hornes M., Frijters A., Pot J., Peleman J., Kuiper M., Zabeau M., 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research. 23: 4407–4414. DOI: 10.1093/nar/23.21.4407
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