Research article

An effective route for the micropropagation of Medinilla formosana through ovary culture in vitro

Yan Wang, Dai-Di Feng, Xiao-Bai Li, Jian-Ping Chen

Yan Wang
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Dai-Di Feng
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Xiao-Bai Li
Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Jian-Ping Chen
State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. Email: jpchen2001@126.com

Online First: June 29, 2015
Wang, Y., Feng, D., Li, X., Chen, J. 2015. An effective route for the micropropagation of Medinilla formosana through ovary culture in vitro. Annals of Forest Research DOI:10.15287/afr.2015.409


Medinilla formosana Hayata, a beautiful endangered shrub of the family Melastomataceae endemic in Taiwan of China, is recalcitrant for micropropagation in vitro until now. And tissue browning after cutting generally occurred in the tissue culture of Medinilla species was considered to be the greatest obstacle. To avoid the serious cut-browning, immature ovaries in 0.3 cm diameter of M. formosana were taken as initial explants in this study, and its aseptic plantlets were obtained for the first time. The highest survival level (12.7 plantlets per ovary) after 3 months of culture were observed on 1/2 MS supplemented with 0.5 g l-1 active carbon and 0.4 g l-1 casein hydrolysate (pH 5.5). Furthermore, β-mercaptoethanol proved to be an effective inhibitor for its browning response of M. formosana in vitro. And 1/2 MS media supplemented with 6.67 μM BA, 0.11 μM NAA, 0.5 mM β-Met and 0.2 g l-1 AC proved appropriate for its multiplication. Finally rooted plantlets were transplanted into the greenhouse after acclimatization, and the micropropagation system of M. formosana was successfully established. This report first details an efficient method for the in vitro micropropagation of Medinilla plants.


Agarwal S., Kanwar K., 2007. Comparison of genetic transformation in Morus alba L. via different regeneration systems. Plant Cell Rep. 26: 177-185. DOI: 10.1007/s00299-006-0217-3

Bhat S.R., Chandel K.P.S., 1991. Anovel technique to overcome browning in tissue culture. Plant Cell Rep. 10: 358-361. DOI: 10.1007/BF00193159

Boufford D. E., Ohashi H., Huang T. C., Hsieh C. F., Tsai J. L., Yang K. C., Peng C. I., Kuoh C. S., Hsiao A.,2003. Achecklist of the vascular plants ofTaiwan. Flora ofTaiwan, 6: 15-139.

Buendia-Gonzalez L., Orozco-Villafuerte J., Cruz-Sosa F., Chavez-Avila V.M., Vernon-Carter E.J., 2007. Clonal propagation of mesquite tree (Prosopis laevigata Humb. & Bonpl. ex Willd. M.C. Johnston). I.via cotyledonary nodes. In Vitro Cell Dev. Biol. Plant. 43: 260-266. DOI: 10.1007/s11627-007-9027-8

Casteele K. L. V., Dauw-van Keymeulen M. I., Debergh P. C., Maene L. J., Flamée M. C., Van Sumere C. F., 1981. The phenolics and a hydrolysable tannin polyphenol oxidase of Medinilla magnifica. Phytochemistry, 20(5):1105-1112. DOI: 10.1016/0031-9422(81)83036-1

Chyuan J.H., 2006. Improvement of cutting propagative of Medinilla formosana Hayata and M. macnifica. Bulletin of the Hualien District Agricultural Improvement Station, 19: 28.

Debergh P.C., Maene L.J., 1981. Ascheme for commercial propagation of ornamental plants by tissue culture. Sci. Hortic. 14(4): 335-345. DOI: 10.1016/0304-4238(81)90047-9

Dörnenburg H., Knorr D., 1997. Evaluation of elicitor-and high-pressure-induced enzymatic browning utilizing potato (Solanum tuberosum) suspension cultures as a model system for plant tissues. J. Agr. Food Chem. 45(10): 4173-4177. DOI: 10.1021/jf9701603

Joshi P., Dhawan V., 2007. Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay. Biol. Plant. 51: 22-26. DOI: 10.1007/s10535-007-0005-0

Liesebach M., Naujoks G., 2004. Approaches on vegetative propagation of difficult-to-root Salix caprea. Plant Cell Tissue Organ Cult. 79: 239-247. DOI: 10.1007/s11240-004-0665-9

Madhusudhanan K., Rahiman B.A., 2000. The effect of activated charcoal supplemented media to browning of in vitro cultures of Piper species. Biol. Plantarum. 43(2): 297-299. DOI: 10.1023/A:1002729032527

Murashige T., Skoog F., 1962. Arevised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum. 15: 473-497. DOI: 10.1111/j.1399-3054.1962.tb08052.x

Rao I.M., Borrero V., Ricaurte J., Garcia R., Ayarza M.A., 1997. Adaptive attributes of tropical forage species to acid soils. III. Differences in phosphorus acquisition and utilization as influenced by varying phosphorus supply and soil type. J. Plant Nutr. 20(1): 155-180. DOI: 10.1080/01904169709365240

Reinert J., Bajaj Y.P.S., 1977. Applied and fundamental aspects of plant cell, tissue and organ culture. Springer, Berlin. DOI: 10.1007/978-3-662-02279-5

Sarni-Manchado P., Le Roux E., Le Guernevé C., Lozano Y., Cheynier V., 2000. Phenolic composition of litchi fruit pericarp. J. Agri. Food. Chem. 48: 5995-6002. DOI: 10.1021/ jf000815r

Soniya E.V., Sujitha M., 2006. An efficient in vitro propagation of Aristolochia indica. Biol. Plant. 50: 272-274. DOI: 10.1007/s10535-006-0018-0

Tang W., NewtonR.J., 2004. Increase of polyphenol oxidase and decrease of polyamines correlate with tissue browning in Virginiapine (Pinus virginiana Mill.). Plant Sci. 167(3): 621-628. DOI: 10.1016/j.plantsci.2004.05.024

Than Than Van K., Trinh H.,1978. Infrontiers of plant tissue culture (Thorpe, T.A., ed.)Calgary,Alta,Canada.

Thomas T.D., 2008. The role of activated charcoal in plant tissue culture. Biotechnol Adv. 26(6): 618-631. DOI: 10.1016/j.biotechadv.2008.08.003

Wang Y., Cheng Q., Zhu X.Y., Chen L.P., 2011. Studies on reproductive characteristics of an interspecific chimera between Brassica juncea and Brassica oleracea. Plant Cell Tiss Organ Cult. 104: 209-215. DOI: 10.1007/s11240-010-9822-5

Walker J.R. L., Ferrar P.H., 1998. Diphenol oxidases, enzyme-catalysed browning and plant disease resistance . Biotechnology and genetic engineering reviews, 15(1): 457-498. DOI: 10.1080/02648725.1998.10647966

Wardlaw C.W., 1955. Embryogenesis in plants. Methuen, Ltd., London. DOI: 10.5962/bhl. title.5655

Watson L., Dallwitz M.J., 1992 onwards. The families of flowering plants: descriptions, illustrations, identification, and information retrieval. Version: 10th October 2013. http://delta-intkey.com

Werckmeister P., 1971. Light induction of geotropism, and the control of proliferation and growth of Cymbidium in tissue culture. Botanical Gazette. 132(4): 346-350. DOI: 10.1086/ 336601

Wu Z.Y., Peter H.R., Hong D.Y., 1994. Flora ofChina. Science Press (Beijing) &MissouriBotanical Garden(St. Louis), 13: 393-394.

Xiao W., Huang X.L., Huang X., Chen Y.P., Dai X.M., Zhao J.T., 2007. Plant regeneration from protoplasts of Musa acuminate cv. Mas (AA) via somatic embryogenesis. Plant Cell Tiss Organ Cult. 90:191-200. DOI: 10.1007/s11240-007-9241-4

Xu Y.W., Zeng J.W., Zou Y.T., Husaini A.M.,YaoR.Y., Wu D.G., 2011. Combined effect of dark and wounding on regeneration potential of Houttuynia cordata Thunb. leaves. Indian J. Exp. Biol. 49: 540-546.

Yingsanga P., Srilaong V., Kanlayanarat S., Noichinda S., McGlasson W. B., 2008. Relationship between browning and related enzymes (PAL, PPO and POD) in rambutan fruit (Nephelium lappaceum Linn.) cvs. Rongrien and See-Chompoo. Postharvest biology and technology, 50(2):164-168. DOI: 10.1016/j.postharvbio.2008.05.004

Zhang S.Z., 2013 Vegetation Ecology of the Midst of Tajen Experimental Forest Station, Taitung County.[master's thesis.] Pingtung,NationalPingtungUniversityof Science and Technology, 12-18.


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