Research article

Thidiazuron: A potent cytokinin for efficient plant regeneration in Himalayan poplar (Populus ciliata Wall.) using leaf explants

Gaurav Aggarwal, Chhaya Sharma , D.K. Srivastava

Gaurav Aggarwal
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India
Chhaya Sharma
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India. Email: chhaya_uhf@yahoo.co.in
D.K. Srivastava
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India

Online First: July 30, 2012
Aggarwal, G., Sharma, C., Srivastava, D. 2012. Thidiazuron: A potent cytokinin for efficient plant regeneration in Himalayan poplar (Populus ciliata Wall.) using leaf explants. Annals of Forest Research 55(2): 179-188.


Populus species are important resource for certain branches of industry and have special roles for scientific study on biological and agricultural systems. The present investigation was undertaken with an objective of enhancing the frequency of plant regeneration in Himalayan poplar (Populus ciliataWall.). The effect of Thiadizuron (TDZ) alone and in combination with adenine and alpha-Naphthalene acetic acid (NAA) were studied on the regeneration potential of leaf explants. A high efficiency of shoot regeneration was observed in leaf (80.00%) explants on MS basal medium supplemented with 0.024 mg/l TDZ and 79.7 mg/l adenine. Elongation and multiplication of shoots were obtained on Murashige and Skoog (MS) basal medium, containing 0.5 mg/l 6. Benzyl aminopurine (BAP) + 0.2mg/l Indole 3-acetic acid (IAA) + 0.3 mg/l Gibberellic acid (GA3). High frequency root regeneration from in vitro developed shoots was observed on MS basal medium supplemented with 0.10 mg/l Indole 3-butyric acid (IBA). Maximum of the in vitro rooted plantlets were well accomplished to the mixture of sand: soil (1:1) and exhibited similar morphology with the field plants. A high efficiency plant regeneration protocol has been developed from leaf explants in Himalayan poplar (Populus ciliata Wall.). 

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  • Gaurav Aggarwal
  • Chhaya Sharma
  • D.K. Srivastava
  • Gaurav Aggarwal
  • Chhaya Sharma
  • D.K. Srivastava