Research note

DOI: 10.15287/afr.2017.819

Development of genic and genomic microsatellites in Gleditsia triacanthos L. (Fabaceae) using Illumina sequencing

Yawen Wu , Ruhua Zhang, Margaret Staton, Scott E. Schlarbaum, Mark V. Coggeshall, Jeanne Romero-Severson, John E. Carlson, Haiying Liang, Yi Xu, Daniela I. Drautz-Moses, Stephan C. Schuster, Oliver Gailing

Yawen Wu
Michigan Technological University, School of Forest Resources and Environmental Science, 1400 Townsend Drive, Houghton, MI 49931. Email: na@yw.com
Ruhua Zhang
Michigan Technological University, School of Forest Resources and Environmental Science, 1400 Townsend Drive, Houghton, MI 49931, USA
Margaret Staton
Department of Entomology and Plant Pathology, The University of Tennessee, Knoxville, TN 37996, USA
Scott E. Schlarbaum
Department of Forestry, Wildlife and Fisheries, The University of Tennessee, Knoxville, TN 37996-4563, USA
Mark V. Coggeshall
USDA Forest Service, Northern Research Station, Purdue University, West Lafayette, IN 47907, USA
Jeanne Romero-Severson
Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
John E. Carlson
The Department of Ecosystem Science and Management and Department of Plant Science, Pennsylvania State University, University Park, PA 16802, SUA
Haiying Liang
Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA
Yi Xu
Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ 08901, USA
Daniela I. Drautz-Moses
The Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA
Stephan C. Schuster
The Department of Genetics and Biochemistry, Clemson University, Clemson, SC 29634, USA
Oliver Gailing
University of Göttingen, Forest Genetics and Forest Tree Breeding, Büsgenweg 2, 37077 Göttingen, Germany

Online First: June 29, 2017
Wu, Y., Zhang, R., Staton, M., Schlarbaum, S., Coggeshall, M., Romero-Severson, J., Carlson, J., Liang, H., Xu, Y., Drautz-Moses, D., Schuster, S., Gailing, O. 2017. Development of genic and genomic microsatellites in Gleditsia triacanthos L. (Fabaceae) using Illumina sequencing. Annals of Forest Research DOI:10.15287/afr.2017.819


Twenty new polymorphic genic SSRs (EST-SSRs) and 13 genomic SSRs were developed in honeylocust (Gleditsia triacanthos) using Illumina transcriptome and low-coverage genome sequencing. A diversity panel of 40 honeylocust samples covering large parts of the species distribution range was characterized. As expected the level of genetic variation was lower in EST-SSRs than for non-genic genomic SSRs. This is the first report of EST-SSRs for honeylocust. All markers are polymorphic and produce clear single locus amplification products and can be used for genetic diversity and gene flow analyses. The transcriptome sequencing data provide a rich resource for new marker development.


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Supp. Info. 1. EST-SSRs, dinucleotide and trinucleotiderepeats for honeylocust (unigene version 082614)
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Supp. Info. 2. Genomic SSRs
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Supp. Info. 3. Geographic coordinates of Gleditsia triacanthos provenances
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